FIGURE 6.
Rhapontigenin attenuates MRSA‐induced ALI in mice. C57BL/6 male mice, 10–12 weeks, were administered vehicle (10% DMSO in saline) or rhapontigenin (IP) at 10 mg/kg for 1 h prior to MRSA (0.75 × 108/mouse) infection (IT). 18 h later, bronchoalveolar lavage (BAL) and lung tissues were collected and analyzed. (A, B) Representative western blots and pooled densitometric analyses for CYP1A1 protein expression in (A) lung tissue and (B) BAL fluid. BAL was assayed for (C) total protein concentration and (D) neutrophil counts. (E) Lung tissue H&E staining. Lung tissues were scanned with a digital slide scanner at 40× and representative images were taken at 20×. Scale bar: 300 μm. Representative lung sections are shown. Each symbol represents an individual mouse. N = 3–5 mice per group. Data were analyzed using an unpaired t‐test or one‐way ANOVA, *p < .05, **p < .01, ***p < .001, ****p < .0001.