Fig. 1.

OSM inhibits IFN-β production and autocrine signaling in TNBC. A BT-549 cells were infected with lentiviruses encoding OSM or control (Vec). Following selection, cells were assessed via Western blot analysis for OSM and STAT3 phosphorylation. B RNA-sequencing was performed on BT-549-OSM and BT-549-Vec cells and pairwise comparisons of the sequencing data were used to assess interferon-family genes. C Western blot and D qRT-PCR analyses of BT-549-OSM and BT-549-Vec cells assessing IFN-β1, ISGs, IFNARs, and ISGF3. Data represents mean fold changes ± SEM, n = 4. Statistical significance was determined via Welch’s t-tests where *p < 0.05 and **p < 0.01. E GSEA of RNA-sequencing data of BT-549-OSM and BT-549-Vec cells was performed using an experimentally-derived IFN-β metagene signature. A false-discovery rate (FDR) correction was applied to the statistical significance. F E0771 cells were infected with lentiviruses encoding OSM or control (Vec). Following selection, cells were assessed via Western blot analysis for STAT3 phosphorylation. G qRT-PCR and H Western blot analyses of E0771-OSM and E0771-Vec cells assessing Ifn-β1, ISGs, IFNARs, and ISGF3. Data represents mean fold changes ± SEM, n = 4. Statistical significance was determined via t tests where *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001