Fig. 4.

TLR3 is a major mediator of IFN-β production and autocrine signaling. A BT-549 cells were infected with lentiviruses encoding OSM or shRNAs targeting either TLR3 or GFP. Following selection, cells were assessed by qRT-PCR and B Western blot analyses for the efficiency of TLR3 knockdown. Data represents mean fold changes ± SEM, n = 4. Statistical significance was determined via Welch’s t-tests where *p < 0.05. C qRT-PCR and D Western blot analyses of BT-549-shTLR3, BT-549-shGFP, and BT-549-OSM cells assessing IFN-β1, ISGs, IFNARs, and ISGF3. Data represents mean fold changes ± SEM, n = 4. Statistical significance was determined via Welch’s t-tests where *p < 0.05. E E0771 cells were infected with lentiviruses encoding OSM or shRNAs targeting either Tlr3 or GFP. Following selection, cells were assessed by qRT-PCR for efficiency of Tlr3 knockdown. Data represents mean fold changes ± SEM, n = 4. Statistical significance was determined via Welch’s t-tests where **p < 0.01. F qRT-PCR and G Western blot analyses of E0771-shTlr3, E0771-shGFP, and E0771-OSM cells assessing Ifn-β1, ISGs, IFNARs, and ISGF3. Data represents mean fold changes ± SEM, n = 4. Statistical significance was determined via Welch’s t-tests where *p < 0.05 and **p < 0.01. H Transwell migration of BT-549-shTLR3, BT-549-shGFP, and BT-549-OSM cells was assessed for 72 h via Incucyte imager. Data is graphed as mean ± SD, n = 6. Statistical significance was determined via two-way ANOVA with multiple comparisons where ****p < 0.0001. I Transwell migration of E0771-shTlr3 and E0771-shGFP cells was assessed for 84 h via Incucyte imager. Data is graphed as mean ± SD, n = 6. Statistical significance was determined via two-way ANOVA with multiple comparisons where **p < 0.01