Fig. 5.

EMT-inducing factors inhibit IFN-β production and autocrine signaling. A BT-549 cells were infected with lentiviruses encoding OSM, TGF-β1, SNAI1, ZEB1, or a control vector (Vec). Following selection, cells were assessed via western blot for the indicated targets. B qRT-PCR and C Western blot analyses of cells from A assessing IFN-β1, ISGs, IFNARs, and ISGF3. D–F GSEA of RNA-sequencing data from the cells in A was performed using an experimentally-derived IFN-β metagene signature; D BT-549-TGF-β1, E BT-549-SNAI1, F and BT-549-ZEB1 cells were compared to BT-549-Vec cells. A false-discovery rate (FDR) correction was applied to the statistical significance. G qRT-PCR and H Western blot analysis of cells from A assessing TLR3 expression. Data represents mean fold changes ± SEM, n = 4. Statistical significance was determined via Welch’s t-tests where **p < 0.01