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. 2024 Nov 26;30:231. doi: 10.1186/s10020-024-00996-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Potential of CAR macrophages to inhibit angiogenesis in vitro. Note: (A) Enzyme-linked immunosorbent assay (ELISA) measurement of angiogenesis-related cytokine levels (pg/ml). (B) Matrigel tube formation assay using culture medium supernatant from the three groups of macrophages and Raji cell co-culture treated HUVECs, with F-actin marked in green fluorescence. A representative photo from one of three repeated experiments is shown. (C) Quantitative analysis and bar graph of the total length of capillary-like structures formed in the Matrigel tube formation assay. (D) Statistical analysis and bar graph of the number of tube-like structures formed in the Matrigel tube formation assay. Note: Quantitative data are presented as mean ± SEM, with p-values determined by two-tailed Student’s t-test, **, p < 0.01