Fig. 3.

Effect of N-acetyl-l-cysteine (NAC) on green fluorescent protein (GFP) activation in mouse embryonic stem reporter cell lines exposed to β-damascone (β-Dam) or potassium bromate (KBrO₃). The GFP reporter activity fold change was calculated from the mean solvent control values for the six reporter cell lines related to DNA damage [(a, g) Bscl2 and (b, h) Rtkn], oxidative stress [(c, i) Srxn1 and (d, j) Blvrb], protein damage [(e, k) Ddit3], and p53 activation [(f, l) Btg2] after 24 h of exposure to β-Dam or KBrO₃. Each grey solid line for reporter activation indicates the GFP induction threshold (a factor of two). The colored solid and dotted lines represent the values obtained with or without NAC co-treatment, respectively. The closed and open symbols represent the values obtained at concentrations causing more than and less than 25% relative cell survival, respectively. Each result represents the mean and standard error of the mean for three independent experiments