Table 1. Sperm quality analysis, cryosurvival rate, and viability rate.

No	Parameter	Fresh (control)	Warming groups			p-value
			Nakagata	Modified	Kitazato
		n = 48	n = 16	n = 16	n = 16
1	Concentration (million/ml)	3.66 ± 0.23	1.89 ± 0.24	2.81 ± 0.22	3.10 ± 0.34	0.000a) 0.007e) 0.690b) 0.488f) 0.818c) 0.000g) 0.009d)
2	Motility (%)	48.06 ± 2.05	40.63 ± 3.81	36.19 ± 2.53	43.50 ± 2.10	0.014a) 0.515e) 0.000b) 0.034f) 0.009c) 0.012g) 0.341d)
3	Viability (%)	51.98 ± 2.24	45.00 ± 1.75	38.00 ± 3.67	36.50 ± 4.28	0.007a) 0.081e) 0.019b) 0.792f) 0.001c) 0.000 ) 0.096d)
4	Morphology (%)	64.19 ± 1.68	33.19 ± 1.86	41.78 ± 2.37	41.25 ± 2.95	0.000a) 0.029e) 0.000b) 0.948f) 0.000c) 0.000g) 0.014d)
5	Sperm membrane integrity (%)	64.38 ± 1.29	40.25 ± 1.78	53.94 ± 2.19	55.88 ± 1.47	0.000a) 0.000e) 0.023b) 0.470f) 0.124c) 0.000g) 0.000d)
6	Cryosurvival Rate (%)	-	42.38 ± 4.74	54.08 ± 6.73	65.43 ± 7.29	0.168d) 0.266f) 0.015e) 0.049g)
7	Viability Rate (%)	72.13 ± 2.41	69.56 ± 3.92	65.44 ± 4.58	66.69 ± 3.61	0.582a) 0.594e) 0.206b) 0.832f) 0.220c) 0.586g) 0.499d)

Note: Data are presented as mean ± standard error mean (mean±SEM). ^a)The fresh sample vs Nakagata (F*N) group, ^b)the fresh sample versus Modified (F*M) group, ^c)the fresh sample vs Kitazato (F*K) group, ^d)the Nakagata versus Modified (N*M) group, ^e)the Nakagata versus Kitazato (N*K), ^f) Modified versus Kitazato (M*K) group and ^g)Nakagata versus Modified versus Kitazato (N*M*K).