FIGURE 5.

Effect of soluble Siglec-5 Fc on tumor antigen-induced T cell response. 1383i T cells were stimulated with T2 pulsed with tyrosinase peptide. 2 days post-stimulation cells were used for stimulation with MEL624 cancer cell line in the presence of soluble Siglec-5 Fc (10 μg/ml) or control hIgG1 Fc (10 μg/ml) protein. The assay was carried in the presence of Brefeldin A and Monensin. (a) Representative plots and summary of frequency of CD4 T cells expressing IL-2, IFN-γ, and TNF-α at 12hrs post-stimulation. (b) Representative plots and summary of frequency of CD4 T cells expressing CD107a. Statistical analysis: Ratio paired two-tailed t-test; *p < 0·05. Each dot represents an individual donor