FIG. 7.

(A) Sequences of PRE-2 mutants. Footprinting studies demonstrate that PRE-2 contains a potential binding site for a transcription factor (underlined). Two double-stranded oligonucleotides, each containing a single PRE-2 motif with a 3-bp substitution in this footprint, were generated and tested for competition in the band shift assay. (B) PRE-2mt1 and PRE-2mt2 do not compete for binding to the detected protein complex. A single radiolabeled PRE-2 motif was used in a band shift assay to probe PalK and PalF nuclear extracts. Cold competitors were added as indicated at either a 100-fold (+) or a 500-fold (∗) molar excess.