FIG. 9.

Mutation of PRE-2 results in an elevated transcriptional response of the BPV-4 promoter. PRE-2mt1 was introduced into the 80bpTATA promoter construct, generating 80bpmt1. One microgram of 80bpTATA and 80bpmt1 reporter constructs was cotransfected into PalK and PalF cells with 0.1 μg of pCMVHPV16-E2 expression vector. pCMV vector was added so that an equivalent amount of DNA was used in each transfection. Results are expressed as fold transactivation relative to the luciferase activity of each reporter in the absence of E2.