Figure 5.

JAK2 and iNOS cascades are implicated in the anti-inflammatory process of limonoid 2. (A) RAW264.7 cells were pre-treated by limonoid 2 (15, 30 μM) for 4 h and then treated with LPS for 24 h. Protein expression of p-STAT-3, STAT-3, p-JAK-2, and JAK-2 was measured by Western blotting. (B) RAW264.7 cells were pre-treated by limonoid 2 (15, 30 μM) for 4 h and then treated with LPS for 24 h. The expression of eNOS and iNOS proteins was detected by Western blotting. * p < 0.05, ** p < 0.01, compared with the LPS group alone.