Figure 4.

Airway permeability and oxidant stress markers in BAL fluid measured in Pon2^−/− and WT mice 24 h following exposure to filtered air (FA) or 2 ppb ozone (O₃). (A–C) Protein, albumin, and 8-isoprostane measured by ELISA. (D) Nitrite was measured by a fluorometric assay. N = 7 for WT FA; N = 6 for Pon2^−/− FA; N = 10 for WT O₃; and N = 11 for Pon2^−/− O₃ in the protein and albumin assays. N = 7 for WT FA; N = 6 for Pon2^−/− FA; N = 8 for WT O₃; and N = 11 for Pon2^−/− O₃ in the 8-isoprostane assay. N = 4 for WT FA; N = 6 for Pon2^−/− FA; N = 4 for WT O₃; and N = 7 for Pon2^−/− O₃ in the nitrite assay. Numbers differ due to availability of BAL fluid sample. Data presented as mean ± standard deviation. Comparison made by 2-way ANOVA. * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001, ns = not significant.