Figure 6.

Mitochondrial Function. Cells were exposed to either 100 µM H₂O₂ or control media for 24 h. 10,000 cells/well were plated in Seahorse XFp plates (n = 3/conditions), and then mitochondrial function was assessed using a MitoStress Test Cartridge. Oxygen Consumption Rate (OCR) is a measure of total electron transport chain function. Extracellular Acidification Rate (ECAR) is a measure of glycolytic flux. (Top Row). No difference was observed in any parameter between the AAVS-1 cells and PON2KD cells under control conditions in both OCR (A) and ECAR (B). (Bottom Row) We observed a reduction in OCR (C) following FCCP addition in PON2KD cells, indicating a decrease in maximal respiration, without changes in baseline respiratory or electron leak. This finding indicates a decrease in mitochondrial space respiratory capacity (SRC). No differences were observed in any ECAR (D) parameter, indicating that changes in OCR were not due to an increase in glycolysis. Comparisons made by 2-way ANOVA with correction for multiple, repeated comparisons. Data presented as mean ± standard deviation * p < 0.05.