Table 2.
RT-qPCR gene expression targets. Gene targets are listed with the corresponding NCBI accession used to design assays for the target, along with the primer and probe sequences used and the resulting efficiency (Primer Eff.) from each run.
| Gene | Primer/Probe Sequence (5′–3′) | Primer Eff. (%) |
|---|---|---|
| Ef1-α NM_001124339.1 |
F: GTGAGTTTGAGGCTGGTATCT R: GCTCAGTAGAGTCCATCTTGTT P:/FAM/TGGGAGTGA/ZEN/AACAGCTCATTGTTGGA/3IABkFQ |
Run 1: 95.95 Run 2: 105.27 |
| β-actin NM_001124235.1 |
F: CTTCTCTCTCCACCTTCCAAC R: GGGATGGGTACAGTCTGTTTAG P:/FAM/CCTCCATCG/ZEN/TCCACCGTAAATGCT/3IABkFQ |
Run 1: 104.63 Run 2: 104.11 |
| TNF-α NM_001124357.1 |
F: CTGGGCTCTTCTTCGTTTACA R: GAGTCCGAATAGCGCCAAATA P:/FAM/AGGCTTCGT/ZEN/TTAGGGTCAAGTGCA/3IABkFQ |
Run 1: 105.46 Run 2: 106.68 |
| NRF-2α XM_036959401.1 |
F: GCAAGCTCATACTCTAGCTCTC R: CAGGGTTACTGTCCATCTCATC P:/FAM/TCCTTTGGT/ZEN/GGCTACAGCGATTCA/3IABkFQ |
Run 1: 94.17 Run 2: 95.91 |
| Ictacalcin S100I2 XM_036967731.1 |
F: GCTTGGAGAGATCATGGGGAAAA R: TCCACACTGCCATCTGCATTAG P:/FAM/ACACTGACC/ZEN/AGGCAAAGGTTGACA/3IABkFQ |
Run 1: 102.58 Run 2: 107.31 |
| SOD BT074393 [51] |
F: CCACGGAGGACCCACTG R: CAGCTCCTGCAGTCACGTT P:/FAM/ACGTGCCGAACAGCAT/NFQ |
Run 1: 99.67 Run 2: 106.18 |
| CAT XM_021564310 [51] |
F: GGACCTTACTGGCAACAACAC R: CGCTTCTGAGAGTGGATAAAGGAT P:/FAM/ACAGCATGGCGTCCCT/NFQ |
Run 1: 95.23 Run 2: 99.44 |
| GPX-1 NM_001124525.1 [52] |
F: CGCCCACCCACTGTTTGT R: GCTCGTCGCTTGGGAATG |
Run 1: 112.31 Run 2: 100.68 |