Table 5.
Target genes for pathogenic E. coli characterization [26].
| PCR | Primer | Nucleotide Sequence | Strain | Size |
|---|---|---|---|---|
| Set-1 | ||||
| pCVD432 | CTGGCGAAAGACTGTATCAT | EAEC | 194 bp | |
| AAATGTATAGAAATCCGCTGTT | ||||
| LT | ACGGCGTTACTATCCTCTC | ETEC | 273 bp | |
| TGGTCTCGGTCAGATATGTG | ||||
| STp | TCTTTCCCCTCTTTTAGTCAG | ETEC | 166 bp | |
| ACAGGCAGGATTACAACAAAG | ||||
| STh | TTCACCTTTCCCTCAGGATG | ETEC | 120 bp | |
| CTATTCATGCTTTCAGGACCA | ||||
| Eae | TCAATGCAGTTCCGTTATCAGTT | EPEC/EHEC | 482 bp | |
| GTAAAGTCCGTTACCCCAACCTG | ||||
| Bfp | GGAAGTCAAATTCATGGGGGTAT | Typical EPEC | 300 bp | |
| GGAATCAGACGCAGACTGGTAGT | ||||
| Set-2 | ||||
| Eae | TCAATGCAGTTCCGTTATCAGTT | EPEC/EHEC | 482 bp | |
| GTAAAGTCCGTTACCCCAACCTG | ||||
| CadA | TTCAAAAACATCGATAACGA | EIEC | 680 bp | |
| ACGGTATGCACCGTGAAT | ||||
| Stx-1 | CAGTTAATGTGGTGGCGAAGG | EHEC | 384 bp | |
| CACCAGACAATGTAACCGCTG | ||||
| Stx-2 | ATCCTATTCCCGGGAGTTTACG | EHEC | 584 bp | |
| GCGTCATCGTATACACAGGAGC | ||||
| VTcom | GAGCGAAATAATTTATATGTG | EHEC | 518 bp | |
| TGATGATGGCAATTCAGTAT |