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. 2024 Nov 7;14(11):540. doi: 10.3390/bios14110540

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) QD-based single-molecule biosensor for monitoring lncRNAs by coupling PER with isothermal circular strand-displacement polymerization reaction [102]. (B) Single-molecule biosensor for the simultaneous quantification of multiple lncRNAs by utilizing magnetic separation techniques and enzyme-free strand displacement reaction [89]. (C) Single-molecule biosensor for quantitative analysis of miRNA assay by integrating CRISPR/Cas13a with DNA-PAINT [100]. (D) Single-molecule biosensor for one-step detection of miRNAs in lung cancer tissues by utilizing PER and CRISPR-Cas12a [101].