Figure 1.

Primary cultures of DRGs were prepared and treated for 24 h or 7 days in hypoxia (hypoxia to mimic endogenous conditions and prevent levodopa auto-oxidation). Cultures were then examined for mitochondrial membrane potential (tetramethylrhodamine, methyl ester (TMRM)), reactive oxygen species (ROS) using dihydroethidium, beta III tubulin and lysosome content using Lysotracker red (red dots in the green DRG soma) and lysosome acidity (Lysotracker red + Lysosensor green, red + green = yellow dots in green DRG soma) as detailed in the text. Cells from the 50B11 cell line were also treated, then cultured in hypoxia for 24 h, and lysosome content was examined, as detailed in the text, using Lysotracker (red dots in the cell soma at top right). Figure made using BioRender.