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. 2024 Nov 17;14(11):1454. doi: 10.3390/biom14111454

Figure 4.

Figure 4

Digested Chaga extract (MW < 3500 Da) induced cell cycle arrest in the G0/G1 phase in SK-BR-3 cells. (A) Histograms showing the percentage of SK-BR-3 cells in the G0/G1, S, and G2/M phases in control condition or following 24 h treatment with 0.5 mg/mL of digested Chaga extract (MW < 3500 Da) as assessed by FACS cell cycle analysis. Data are presented as the mean ± SD of three repeats. **** p < 0.0001 vs. control. ANOVA followed by Sidak’s multiple comparison test. (B) Representative Western blotting showing the expression of CDK4, cyclin D1, cyclin E2, phosphorylated Rb protein (Ser 780), and β-actin (loading control) in SK-BR-3 cells left untreated (Ctrl) or treated with 0.5 mg/mL or 1 mg/mL of digested Chaga extract (MW < 3500 Da) for 24 h or 48 h. Twenty micrograms of proteins/well were loaded. (C) Densitometric analysis of each assessed protein. Data are presented as the mean ± SE of three repeats. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns: not significant. One-way ANOVA followed by Dunnet’s multiple comparison test.