Figure 6.

Digested Chaga extract inhibited DHFR enzymatic activity in BC cells. Residual enzymatic activity of DHFR was analyzed in SK-BR-3 (A) and MDA-MB-231 (B) cells after a 4–6 h treatment with 0.5 mg/mL or 1 mg/mL digested Chaga extract (MW < 3500 Da). (C) The residual enzymatic activity of DHFR (as % of the control) is reported in the presence of Chaga extract 1 mg/mL in comparison with the residual activity obtained in the presence of the mixture of digestive fluids used to prepare the Chaga extract at 1 mg/mL. Data are reported as the average of three replicates ± SE, ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. ns: not significant. One-way ANOVA followed by Tukey’s multiple comparison test.