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Digested Chaga extract (MW < 3500 Da) induced cytokine release in the culture medium of both SK-BR-3 and MDA-MB-231 cultures but differently according to the cell line. Cytokines were quantified by using a multiplex immunoassay, as described in Materials and Methods section. Data were analyzed by Student’s t-test. * p < 0.05, ** p < 0.01, **** p < 0.0001, with respect to the corresponding Ctrl supernatant cytokine concentration.
