FIG. 5.

v-FLIP protein expression in KSHV-infected B cells. (A) Western blot of 70 μg of protein from the EBV-transformed B.45 cells and the KSHV-transformed EBV-negative PEL BC3 and BCP1 cells and protein from BC3 cells uninduced or induced for 48 h with 200 ng of tetradecanoyl phorbol acetate (TPA)/ml and separated by SDS–14% PAGE. Immunoblotting with a polyclonal anti-v-FLIP antiserum was performed as described in Materials and Methods. (B) Western blot of 70 μg of protein from EBV-negative B-cell line DG75 and the KSHV-transformed PEL BC3 cells separated by SDS–14% PAGE. Immunoblotting with monclonal anti-v-FLIP antibody 6/14 was performed as described in Materials and Methods.