Figure 1.

IKE and Eto combined treatment increased MDSCs’ actual ferroptotic death in vitro. Flow cytometry detected the percentages of PI⁻ cell viability and fold change of indicated treatment relative to control group (a), mean fluorescence intensity (MFI) of ROS production and fold change of indicated treatment relative to control group (b), MFI of fatty acid uptake and fold change of indicated treatment relative to control group (c), MFI of lipid droplets and fold change of indicated treatment relative to control group (d), MFI of lipid peroxidation and fold change of indicated treatment relative to control group (e), MFI of mitochondrial superoxide anions and fold change of indicated treatment relative to control group (f), MFI of mitochondrial Fe²⁺ and fold change of indicated treatment relative to control group (g), and MFI of mitochondrial mass and fold change of indicated treatment relative to control group (h) in in vitro BM-MDSCs. Results from three independent experiments (a–h). Statistical analysis was performed using one-way ANOVA (a–h). Data are expressed as means ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; ns, no significant difference.