Figure 2.
The spot test and colony count analyses show increased sensitivity to LiCl for the caf20∆ and ecm32∆ yeast strains. As shown in (A), the yeast cells were serially diluted (10−1 to 10−4) and spotted onto Ypgal media. In (B), as in A, but the cells were spotted onto Ypgal medium with 10 mM LiCl. The gene deletion mutants caf20∆ and ecm32∆ had reduced growth in the presence of LiCl, with tif2∆ being used as a control. Phenotypic rescue was observed in mutant strains with overexpressed TIF2 or when the respective target gene was restored. (C) The result of colony count analysis. The deletion mutant strains significantly reduced colony counts in the presence of LiCl compared with the wild-type. Mutant strains with overexpression plasmids had colony counts similar to the wild-type. p-Values of tif2∆ = 4.89 × 10−5, caf20∆ = 7.53 × 10−5, and ecm32∆ = 2.65 × 10−5 were obtained, with all other results being insignificant. Letters above the bars indicate statistical significance. Bars that share the same letter do not have a significant difference between them, while bars with different letters have a significant difference between them. The experiments were performed in triplicate, and the data are presented as the mean values. Bars represent the standard deviations (SDs). Statistical analysis was performed by using one-way analysis of variance (ANOVA), with multiple comparisons followed by Tukey’s post hoc test. A p-value < 0.05 was considered to indicate statistical significance.