Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1997 Jan 15;498(Pt 2):497–501. doi: 10.1113/jphysiol.1997.sp021875

Evidence for uncoupling of oxygen and glucose utilization during neuronal activation in rat striatum.

J P Lowry 1, M Fillenz 1
PMCID: PMC1159218  PMID: 9032696

Abstract

1. Changes in regional cerebral blood flow (rCBF), tissue oxygen and extracellular glucose were measured during neuronal activation, using implanted electrodes in the striatum of freely moving rats. 2. There was a parallel increase in rCBF and oxygen in response to neuronal activation. 3. During the neuronal activation there was a decrease in extracellular glucose; following neuronal activation there was a slow rise in extracellular glucose which took 30 min to return to basal levels. 4. The implications of the different time courses of these changes are discussed.

Full text

PDF
501

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baron J. C., Lebrun-Grandie P., Collard P., Crouzel C., Mestelan G., Bousser M. G. Noninvasive measurement of blood flow, oxygen consumption, and glucose utilization in the same brain regions in man by positron emission tomography: concise communication. J Nucl Med. 1982 May;23(5):391–399. [PubMed] [Google Scholar]
  2. Boutelle M. G., Zetterström T., Pei Q., Svensson L., Fillenz M. In vivo neurochemical effects of tail pinch. J Neurosci Methods. 1990 Sep;34(1-3):151–157. doi: 10.1016/0165-0270(90)90053-i. [DOI] [PubMed] [Google Scholar]
  3. Fellows L. K., Boutelle M. G., Fillenz M. Physiological stimulation increases nonoxidative glucose metabolism in the brain of the freely moving rat. J Neurochem. 1993 Apr;60(4):1258–1263. doi: 10.1111/j.1471-4159.1993.tb03285.x. [DOI] [PubMed] [Google Scholar]
  4. Fellows L. K., Boutelle M. G. Rapid changes in extracellular glucose levels and blood flow in the striatum of the freely moving rat. Brain Res. 1993 Feb 26;604(1-2):225–231. doi: 10.1016/0006-8993(93)90373-u. [DOI] [PubMed] [Google Scholar]
  5. Forsyth R., Fray A., Boutelle M., Fillenz M., Middleditch C., Burchell A. A role for astrocytes in glucose delivery to neurons? Dev Neurosci. 1996;18(5-6):360–370. doi: 10.1159/000111429. [DOI] [PubMed] [Google Scholar]
  6. Fox P. T., Raichle M. E. Focal physiological uncoupling of cerebral blood flow and oxidative metabolism during somatosensory stimulation in human subjects. Proc Natl Acad Sci U S A. 1986 Feb;83(4):1140–1144. doi: 10.1073/pnas.83.4.1140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fox P. T., Raichle M. E., Mintun M. A., Dence C. Nonoxidative glucose consumption during focal physiologic neural activity. Science. 1988 Jul 22;241(4864):462–464. doi: 10.1126/science.3260686. [DOI] [PubMed] [Google Scholar]
  8. Fray A. E., Forsyth R. J., Boutelle M. G., Fillenz M. The mechanisms controlling physiologically stimulated changes in rat brain glucose and lactate: a microdialysis study. J Physiol. 1996 Oct 1;496(Pt 1):49–57. doi: 10.1113/jphysiol.1996.sp021664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jueptner M., Weiller C. Review: does measurement of regional cerebral blood flow reflect synaptic activity? Implications for PET and fMRI. Neuroimage. 1995 Jun;2(2):148–156. doi: 10.1006/nimg.1995.1017. [DOI] [PubMed] [Google Scholar]
  10. Lowry J. P., Boutelle M. G., O'Neill R. D., Fillenz M. Characterization of carbon paste electrodes in vitro for simultaneous amperometric measurement of changes in oxygen and ascorbic acid concentrations in vivo. Analyst. 1996 Jun;121(6):761–766. doi: 10.1039/an9962100761. [DOI] [PubMed] [Google Scholar]
  11. Morrison P. F., Bungay P. M., Hsiao J. K., Ball B. A., Mefford I. N., Dedrick R. L. Quantitative microdialysis: analysis of transients and application to pharmacokinetics in brain. J Neurochem. 1991 Jul;57(1):103–119. doi: 10.1111/j.1471-4159.1991.tb02105.x. [DOI] [PubMed] [Google Scholar]
  12. Morton D. B., Griffiths P. H. Guidelines on the recognition of pain, distress and discomfort in experimental animals and an hypothesis for assessment. Vet Rec. 1985 Apr 20;116(16):431–436. doi: 10.1136/vr.116.16.431. [DOI] [PubMed] [Google Scholar]
  13. Sokoloff L. The brain as a chemical machine. Prog Brain Res. 1992;94:19–33. doi: 10.1016/s0079-6123(08)61736-7. [DOI] [PubMed] [Google Scholar]
  14. Tsacopoulos M., Magistretti P. J. Metabolic coupling between glia and neurons. J Neurosci. 1996 Feb 1;16(3):877–885. doi: 10.1523/JNEUROSCI.16-03-00877.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Zimmerman J. B., Wightman R. M. Simultaneous electrochemical measurements of oxygen and dopamine in vivo. Anal Chem. 1991 Jan 1;63(1):24–28. doi: 10.1021/ac00001a005. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES