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. 2024 Nov 11;14(11):1434. doi: 10.3390/biom14111434

Figure 5.

Figure 5

Impacts of NTI-164 and CBD on SHSY-5Y neurons exposed only to excipients (control, white), excipient + NTI164 (+NTI164, grey), excipient + CBD (+CBD, black) excipient + excitotoxicity (Excitotoxicity, pink) and excitotoxicity + NTI164 (+NTI164, grey hatched) and excitotoxicity + CBD (+CBD, black hatched). (A) Impacts on the number of DAPI-positive cells in undifferentiated cultures; (B) impacts on the expression of beta-tubulin (BTUB) as a proportion of DAPI-positive cells as an indicator of spontaneous differentiation, with representative images in (C). (D) Impacts on the number of DAPI-positive cells in differentiated cultures. (E) Impacts on the expression of BTUB in differentiated cells, with representative images in (F). (G) The impacts on the MTT output (mitochondrial activity assay) in differentiated cells in the glutamate exposure excitotoxicity assay. Scale bar = 150 µM, same across all panels. Mean ± SEM, n = 3–10. One-way ANOVA with post hoc test as indicated on the graph. Significance set at **, p < 0.01; ****, p < 0.0001. ns = p > 0.05.