Figure 11.

High throughput and in situ analysis of lectin interacting with membrane proteins with ESM. (A) Bright field and ESM image of live A431 cells. (B) The image intensity variation against time achieved by averaging the signal of all cells within the field of view. (C) Zoomed views of marked region at 0 s and 216 s after changing the flow to WGA solution, and the differential image. (D) The image intensity variation against time achieved from the cell in the marked zone in (A). (E–H) Statistical distributions of association rate constant, dissociation rate constant, dissociation constant and maximum response value in the binding curves achieved from the individual cells, reprinted with permission from Ref. [44].