FIG. 4.

Differential fiber-dependent adenoviral transduction properties of HeLa cells with pseudotyped Ad5 vectors. HeLa cells were transduced with 1,000 total particles of Ad5.βgal.ΔF/F⁺ per cell pseudotyped with the indicated fiber or were mock transduced (−). After 24 h, the cells were analyzed for β-galactosidase activity by using a chemiluminescence reporter assay. The β-galactosidase activity of each pseudotyped adenoviral vector containing a mutated fiber protein is expressed as a percentage of the activity measured for Ad5.βgal.ΔF/F⁺ pseudotyped with a wild-type fiber protein (wt). All values are derived from five to six separate transductions, each of which was carried out in triplicate, and are presented as the mean percentage of that of the wild type ± standard deviation. ∗, significantly different from wild-type fiber according to an unpaired, two-tailed t test analysis (P < 0.001).