Differential fiber-dependent adenoviral transduction properties of HeLa cells. HeLa cells were transduced with 1,000 total particles per cell with the indicated pseudotyped adenoviral vectors: F−, wild type (wt), KO1, KO2a, KO2, KO2c, KO4, and KO10. After 24 h, the cells were analyzed for β-galactosidase expression by staining the monolayers with X-Gal as described in Materials and Methods. Representative photomicrographs are shown.