TABLE 1.
Oligonucleotides used in Ad5 fiber gene mutagenesis
| Fiber expression plasmid | Oligonucleotide sequence | Fiber mutationa |
|---|---|---|
| pDKO1 | 5′-ACCACACCAGCTCCAGAGGCTAACTGTAGACTAAATGC-3′ | S408E P409A |
| 5′-GCATTTAGTCTACAGTTAGCCTCTGGAGCTGGTGTGTT-3′ | ||
| pDKO2 | 5′-ACAGTTTCAGTTTTGGCCGGCAGTTTGGCTCCAATATC-3′ | ΔV441 K442 |
| 5′-GATATTGGAGCCAAACTGCCGGCCAAAACTGAAACTGT-3′ | ||
| pDKO2a | 5′-ACAGTTTCAGTTTTGGCTAAAGGCAGTTTGGCTCCA-3′ | ΔV441 |
| 5′-TGGAGCCAAACTGCCTTTAGCCAAAACTGAAACTGT-3′ | ||
| pDKO2b | 5′-GTTTCAGTTTTGGCTGTTGGCAGTTTGGCTCCAATA-3′ | ΔK442 |
| 5′-TATTGGAGCCAAACTGCCAACAGCCAAAACTGAAAC-3′ | ||
| pDKO2c | 5′-GTTTCAGTTTTGGCTGCTGCAGGCAGTTTGGCTCCA-3′ | V441A K442A |
| 5′-TGGAGCCAAACTGCCTGCAGCAGCCAAAACTGAAAC-3′ | ||
| pDKO3 | 5′-GCTCATCTTATTATAGAATTCGACGAAAATGGAGTG-3′ | R460E |
| 5′-CACTCCATTTTCGTCGAATTCTATAATAAGATGAGC-3′ | ||
| pDKO4 | 5′-GCTTATCCAAAATCTCACACTGCCAAAAGTAACATTGTC-3′ | ΔG509 K510 |
| 5′-GACAATGTTACTTTTGGCAGTGTGAGATTTTGGATAAGC-3′ | ||
| pDKO5 | 5′-CTAACCATTACACTAAACCAGGAAACAGGAGACAC-3′ | ΔG538 T539 |
| 5′-GTGTCTCCTGTTTCCTGGTTTAGTGTAATGGTTAG-3′ | ||
| pDKO8 | 5′-ATAAGATTTGACGAAACTGGAGTGCTACTAAAC-3′ | N464T |
| 5′-GTTTAGTAGCACTCCAGTTTCGTCAAATCTTAT-3′ | ||
| pDKO9 | 5′-TTTGACGAAAATGGACACCTACTAAACAATTCC-3′ | V466H |
| 5′-GGAATTGTTTAGTAGGTGTCCAGTTTCGTCAAA-3′ | ||
| pDKO10 | 5′-AACCTATCAGCTTATGCAAAATCTCACGGTAAA-3′ | P505A |
| 5′-TTTACCGTGAGATTTTGCATAAGCTGATAGGT-3′ |
a
Amino acid residues are numbered according to the system of Xia et al. (42).