TABLE 2.
Transduction efficiency of pseudotyped Ad5.βgal.ΔF/F+ on HeLa cells
| Fiber expression plasmid | Fiber mutant designation | Fiber mutant mutationa | Representative avg β-galactosidase activity (RLU/μg of protein)b | Mean % of wild-type activity (± SD)c |
|---|---|---|---|---|
| pDV60 | Wild type | None | 530,000 | 100 (±0.9) |
| pDV55 | F− | Null | 266 | 0.1 (±0.1)* |
| pDKO1 | KO1 | S408E P409A | 7,620 | 1.4 (±0.8)* |
| pDKO1+2 | KO1+2 | S408E P409E ΔV441 K442 | 472 | 0.1 (±0.0)* |
| pDKO2 | KO2 | ΔV441 K442 | 3,320 | 0.6 (±0.2)* |
| pDKO2a | KO2a | ΔV441 | 4,000 | 0.6 (±0.2)* |
| pDKO2b | KO2b | ΔK442 | 44,400 | 8.3 (±0.6)* |
| pDKO2c | KO2c | V441A K442A | 53,300 | 8.5 (±2.2)* |
| pDKO3 | KO3 | R460E | 359,000 | 63.3 (±9.1)* |
| pDKO4 | KO4 | ΔG509 K510 | 213,000 | 38.2 (±2.8)* |
| pDKO5 | KO5 | ΔG538 T539 | 331,000 | 58.3 (±7.1)* |
| pDKO4+5 | KO4+5 | ΔG509 K510 ΔG538 T539 | 500,000 | 91.1 (±12.1) |
| pDKO8 | KO8 | N464T | 470,000 | 92.6 (±16.9) |
| pDKO9 | KO9 | V466H | 391,000 | 80.9 (±15.1) |
| pDKO10 | KO10 | P505A | 447,000 | 79.6 (±6.2) |
| pDKO11 | KO11 | Δ404-581 | 4,520 | 0.8 (±0.1)* |
a
Fiber amino acid residues are numbered according to the system of Xia et al. (42).
b
Each value represents the average of three wells.
c
Mean (± standard deviation [SD]) of the β-galactosidase activity of Ad5.βgal.ΔF/F+ pseudotyped with each corresponding fiber mutant in five to six separate transductions. All values were normalized to that of the wild type at 100%. *, significantly different from wild-type fiber activity according to an unpaired, two-tailed t test analysis (P < 0.001).