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. 2001 Mar;75(6):2982–2992. doi: 10.1128/JVI.75.6.2982-2992.2001

TABLE 2.

Cell surface expression of CD8-EnvCD hybrids in HeLa cellsa

Vector pCD8 % GFP+ CD8+ cellsb Mean CD8 fluorescence intensityc Pd
NTe 0.4 ± 0.1 1.4 ± 0.1
EnvCD wt 41.7 ± 4.1 4.5 ± 0.6
LL855/856AA 49.4 ± 6.5 5.3 ± 0.7 >0.05
Y712A 60.2 ± 2.1 6.1 ± 0.3 <0.05
Y712A-LL855/856AA 68.4 ± 1.0 8.4 ± 0.3 <0.05
a

Values are the averages of three independent experiments. After 48 h, surface expression of CD8 was analyzed in GFP+ cells by flow cytometry. 

b

Percentage of GFP+ cells expressing CD8 hybrids at the cell surface. Values are means ± SEM. 

c

Means of CD8 fluorescence intensity of GFP+ cells expressing the CD8 hybrid at the cell surface ± SEM. 

d

The results for the mutant CD8-EnvCD hybrids were compared to those for the wild type by means of Student's t test for unpaired samples. Data obtained with the double mutant (tyrosine and dileucine) were compared to those obtained with the CD8-Y712A chimera. 

e

NT, the pRSV-GFP vector alone was used for cell transfection.