TABLE 2.
Cell surface expression of CD8-EnvCD hybrids in HeLa cellsa
| Vector pCD8 | % GFP+ CD8+ cellsb | Mean CD8 fluorescence intensityc | Pd |
|---|---|---|---|
| NTe | 0.4 ± 0.1 | 1.4 ± 0.1 | |
| EnvCD wt | 41.7 ± 4.1 | 4.5 ± 0.6 | |
| LL855/856AA | 49.4 ± 6.5 | 5.3 ± 0.7 | >0.05 |
| Y712A | 60.2 ± 2.1 | 6.1 ± 0.3 | <0.05 |
| Y712A-LL855/856AA | 68.4 ± 1.0 | 8.4 ± 0.3 | <0.05 |
a
Values are the averages of three independent experiments. After 48 h, surface expression of CD8 was analyzed in GFP+ cells by flow cytometry.
b
Percentage of GFP+ cells expressing CD8 hybrids at the cell surface. Values are means ± SEM.
c
Means of CD8 fluorescence intensity of GFP+ cells expressing the CD8 hybrid at the cell surface ± SEM.
d
The results for the mutant CD8-EnvCD hybrids were compared to those for the wild type by means of Student's t test for unpaired samples. Data obtained with the double mutant (tyrosine and dileucine) were compared to those obtained with the CD8-Y712A chimera.
e
NT, the pRSV-GFP vector alone was used for cell transfection.