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. 2024 Nov 10;13(22):1862. doi: 10.3390/cells13221862

Figure 2.

Figure 2

In vitro efficacy of CD47 antibody in osteosarcoma cells. (A) The expression of CD47 is analyzed using FACS analysis in osteosarcoma cells. Representative images display the histogram of CD47 expression under basal conditions. (B–D) Osteosarcoma cells are treated with either isotype control or anti-CD47 (B6H12) antibody at the indicated concentrations for 24, 48, and 72 h. The effect of the anti-CD47 antibody on osteosarcoma cell viability is assessed using the EZ-CyTox cell viability assay kit. (E) KHOS/NP osteosarcoma cells are exposed to an isotype control or anti-CD47 (B6H12) antibody at the indicated concentrations for 24, 48, and 72 h. The effect of the anti-CD47 antibody on KHOS/NP cell apoptosis is evaluated using the Annexin V Detection kit.