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. 2024 Nov 10;13(22):1862. doi: 10.3390/cells13221862

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Wound-healing assay to assess migration of osteosarcoma cells following CD47 (B6H12) antibody treatment. (A) MG-63 osteosarcoma cells are treated with a 10 μg/mL concentration of an isotype control antibody or the anti-CD47 antibody (B6H12). Representative images show the area the cells cover at the indicated time points after in vitro scratch wounding. (B) The summary graph illustrates the percentage of wound closure in MG-63 osteosarcoma cells over time compared with untreated controls. Statistical analysis of wound closure is conducted using ImageJ software with the wound healing size plug-in. The percentages are represented as the mean ± standard error of the mean, and statistical significance is determined by comparison with the control (* p < 0.05).