Table 1.
Plasmids and strains in this study.
| Strains | Description | References |
|---|---|---|
| E. coli | ||
| DH5α | supE44, ΔlacU169 (φ80 lacZΔM15), hsdR17 (rk-mk+), recA1, endA1, thi1, gyrA, relA | Tiangen |
| S17-1 | RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510 | Tiangen |
| P. putida | ||
| QSRZ6 | KT2440∆hsdR-∆phaZ | Lab stock |
| QSRZ601 | QSRZ6∆gcd | This study |
| QSRZ602 | QSRZ6∆gltA | This study |
| QSRZ603 | QSRZ6∆gcd-∆gltA | This study |
| QSRZ604 | QSRZ6∆hexR | This study |
| QSRZ605 | QSRZ6∆gcd-∆hexR | This study |
| QSRZ606 | QSRZ6∆gltA-∆hexR | This study |
| QSRZ607 | QSRZ6 ∆gcd-∆gltA-∆hexR | This study |
| QSRZ608 | QSRZ605::P17-gltB | This study |
| QSRZ609 | QSRZ605::P33-gltB | This study |
| QSRZ610 | QSRZ605::P33-gltB::P33-phaD | This study |
| Plasmids | ||
| pK18mobsacB | Mobilizable vector, KmR, sacB | Lab stock |
| pK18-∆gcd | A derivative of pK18mobsacB, harboring homology arms of gcd | This study |
| pK18-∆gltA | A derivative of pK18mobsacB, harboring homology arms of gltA | This study |
| pK18-∆hexR | A derivative of pK18mobsacB, harboring homology arms of hexR | This study |
| pK18-P17-gltB | A derivative of pK18mobsacB, harboring P17-gltB fragment and homology arms | This study |
| pK18-P33-gltB | A derivative of pK18mobsacB, harboring P33-gltB fragment and homology arms | This study |
| pK18-P33-phaD-∆hexR | A derivative of pK18mobsacB, harboring P33-gltB fragment and homology arms of hexR | This study |