Figure 2.

Assessment of MGMT gene promoter hypermethylation using the ddPCR technique. (A) Scheme of ddPCR: DNA amplification occurs independently in thousands of drops through a water-in-oil emulsion. Droplets are classified as positive or negative based on the emitted fluorescence wavelength. (B) Linearity of quantification of ultramer oligonucleotide mixture assessed by ddPCR. (C) Methylation analysis by ddPCR using three independent bisulfite treatments in nine different mCRC samples. (D) MGMT methylation profiling of CRC tissue samples obtained by ddPCR.