Figure 7.

PDAC chemoresistant cell differential mechanobiology does not rely on YAP nuclear translocation nor on EMT switch. (A) Relative gene expression of E-cadherin, N-cadherin, and Vimentin as assessed by RT-qPCR. Data are expressed as the mean ± SD of three independent experiments. (B) Representative confocal microscopy images of SUIT-2.028 cells growing on soft (11 kPa) pillars and stained with YAP. Scale bar: 10 µm (C) YAP nuclear translocation, expressed as % of nuclear YAP over total YAP in SUIT-2.028 and SUIT-2.007. (D) Linear regression model between the mean force per pillar vs. % of nuclear YAP in SUIT-2.007 (left panel) and SUIT-2.028 (right panel). Each dot in (C,D) represents one cell. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001, “ns” means not significant.