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Microcapillary electrophoretograms of RT-PCR amplicons produced with primers designed to anneal to exon 3 and exon 5 of SERAC1. The RNA from a CMSD-affected (lane 4) and two unaffected (lanes 2 and 3) Chinese Crested dogs. A band at 253 bp was expected from the consecutive splicing of exon 3 to exon 4 to exon 5. A band at 216 bp was expected from the splicing of exon 3 to exon 5. Lane 1 is a no-template control (lane 1). The band at 500 bp is from an internal molecular weight marker.
