Figure 6.

(A) Results of cell viability (MTT cytotoxicity test) and (B) cell proliferation (measured using resazurin protocol) biological assays for the porous three-dimensional structures (3d = 3 days). (C) Calcein AM (viable cells, green fluorescence, left side images, identified as LIVE) and ethidium homodimer (dead cells, red fluorescence, right side images, identified as DEAD) staining for keratinocyte and fibroblast monoculture cells deposited onto the lower and upper faces of the L-arginine functionalized 3D constructs, respectively (scale bar = 100 µm). Details: schematic representation of cell deposition (not to scale).