Table 3.
Bacterial strains and plasmids were used in this study.
| Strain/Plasmid | Description and Use | Source or Reference |
|---|---|---|
| BL24 | Natural isolate from a soil sample taken near Yantra River’s bed near Veliko Tarnovo, Bulgaria (43°04′52.46″ N 25°37′44.54″ E). Used as a host for sacB gene disruption. | [18,36] |
| E. coli STELLARTM | F-, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, Φ80d lacZΔ M15, Δ(lacZYA-argF) U169, Δ(mrr-hsdRMS-mcrBC), ΔmcrA, λ-. Used as a host in cloning procedures. | Takara Bio Company (Mountain View, CA, USA) |
| pBacTag-DYKDDDDK | B. subtilis chromosomal integration vector; EryR, AmpR, Epitope FLAG tag. Used for ΔsacB disruption cassette construction. | MoBiTec GmbH, Goettingen, Germany |
| pCR®2.1-TOPO® | E. coli TOPO-TA cloning vector. Used as a source of the KanR (NeoR) gene. | Thermo Fisher Scientific Inc., Waltham, MA, USA |
| BLΔsacB | A mutant of BL24 containing sacB gene knockout. Used for 2,3-BD production. | This study |
| pBac_Kan | Chromosomal integration vector; KanR, AmpR. Used for cloning of ΔsacB PCR fragment. | This study |
| pBac_Kan_ΔsacB | ΔsacB—containing integrative construct. Used for ΔsacB knockout in BL24 chromosomes. | This study |