Table 4.
Nucleotide sequences of the primers used for gene amplification and/or sequencing. Underlined bases = inserted RBS (Ribosome-binding site). Bases in bold = inserted stop codons. Bases in italics = tails for cloning with Gibson Assembly®.
| Primer | Sequence (5′–3′) |
PCR Product | Tm (°C) |
Molecule Size (bp) |
|---|---|---|---|---|
| Bac_F | attctatgagtcgcttttgtaaatt | pBacTag_ΔEryR | 63.8 | 4738 |
| Bac_R | tgtaatcactccttcttaattacaa | 62.4 | ||
| Kan_F | gaaggagtgattacaaaagagaaagcaggtagcttgc | KanR | 65.2 | 1009 |
| Kan_R | agcgactcatagaattcagaagaactcgtcaagaaggcg | 68.5 | ||
| BK_F | ggattataaagatgatgatgataaa | pBacTag_KanR | 59.2 | 5747 |
| BK_R | ggtaccctcgactctagat | 63.4 | ||
| sac_F | gatctagagtcgagggtacctactaatagcaaggagaagactccctattc | ΔsacB | 61.0 | 685 |
| sac_R | ctttataatccggccgaaaattccccgctttattctaag | 62.0 |