Expression of key genes related to the pathway from sucrose unloading to starch synthesis under HT stress. SuS, sucrose synthases; FK, fructokinase; PGI, phosphoglucoisomerase; PGM, phosphoglucomutase; AGP, ADP-glucose pyrophosphorylase; SSS, soluble starch synthase; GBSS, granule-bound starch synthase; SBE, starch branching enzymes; Am, amylose; Ap, amylopectin. (A) Expression patterns of key genes related to the pathway from sucrose unloading to starch synthesis. The colors in the heatmap indicate the expression levels of genes across different samples. (B) qRT-PCR was used to verify the expression profile of selected genes (TraesCS4D02G286500, TraesCS4B02G287800, and TraesCS4A02G016400). Bars represent the SE (n = 3), and the means with different letters are significantly different at p < 0.05.