FIG. 5.

Western blot analysis of the light and heavy subunits of Gal/GalNAc lectin from E. histolytica trophozoites axenically and monoxenically grown with E. coli for 1 month. Western immunoblots were interacted with polyclonal (A) and monoclonal (B) antibodies directed against the heavy and light Gal/GalNAc subunits, respectively. Horseradish peroxidase-conjugated antibodies were used as a secondary antibody and developed by enhanced chemiluminescence. Lanes: 1, axenic HK-9; 2 and 3, HK-9 grown with E. coli O55 and 346, respectively; 4, axenic HM-1; 5, HM-1 grown with E. coli O55. The asterisks indicate the 35-kDa bands that are almost missing in trophozoites grown with E. coli O55.