Total-RNA levels of Gal/GalNAc lectin subunits and amebapore from axenic and E. coli-cocultured E. histolytica trophozoites of strain HM-1:IMSS. Northern hybridization of amebic RNA with labeled DNA probes from actin (A), light (B) and heavy (C) Gal/GalNAc lectin subunits, and amebapore (D) is shown. Lanes: 1, axenic HM-1; 2 and 3, HM-1 grown with E. coli O55 and 346, respectively. The values indicate the optical density of each band, taking the value of axenic HK-9 (1.0) as the reference for each probe.