FIG. 3.

Intracellular growth of icmN and icmM insertion mutants in HL-60-derived macrophages and A. castellanii. (A) Chromosomal arrangement of the region surrounding icmN. The chromosomal arrangements of the mutants tested are shown above the genes. Intracellular growth in HL-60-derived macrophages (B) and in A. castellanii (C) was tested as described in Materials and Methods; the experiments were done at least three times, and results similar to those shown were obtained. ■, JR32; ○, GS3005, □, GS3006, ▵, GS3007; ◊, GS3008.