Figure 7.

Ectopic overexpression of orf116b leads to partial and complete sterility in rice. (A) Subcellular localization of MTS-orf116b in Arabidopsis protoplasts demonstrates its expression within the mitochondria. Green represents the green fluorescent protein (GFP) signal; bright red indicates the mitochondrial localization signal; magenta indicates the autofluorescence of chloroplasts; orange-yellow and orange indicate the overlap of the green fluorescent signal and the red fluorescent signal. Scanning electron microscopic images of anthers in the wild-type rice. Scale bars of (A) correspond to 20 μm. (B1–B3), the partial sterile OE-MTS-orf116b rice (C1–C3), and the completely sterile OE-MTS-orf116b rice (D1–D3). I₂-KI staining of pollen grains of the wild-type rice (B4), the partially sterile OE-MTS-orf116b rice (C4), and the completely sterile OE-MTS-orf116b rice (D4). Panicles of the wild-type rice (B5), the partial sterile OE-MTS-orf116b rice (C5), and the completely sterile OE-MTS-orf116b rice (D5). Seeds of the wild-type rice (B6), the partial sterile OE-MTS-orf116b rice (C6), and the completely sterile OE-MTS-orf116b rice (D6). Scale bars of (B1,C1,D1) correspond to 500 μm; scale bars of (B2,C2,D2) correspond to 100 μm; scale bars of (B3,C3,D3) correspond to 20 μm; scale bars of (B4,C4,D4) correspond to 200 μm.