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. 2024 Nov 14;25(22):12257. doi: 10.3390/ijms252212257

Figure 8.

Figure 8

Overexpression of orf116b causes partial and complete sterility in Arabidopsis, tobacco, and cotton. The main branch of the wild-type (A) and OE-MTS-orf116b transgenic (B) Arabidopsis plants. Alexander staining of the pollen of the wild-type (C) and OE-MTS-orf116b transgenic Arabidopsis plants (D) at the full bloom stage. Pods of the wild-type (E) and OE-MTS-orf116b transgenic Arabidopsis plants (F) at the pod formation stage. Pods of the wild-type (G) and OE-MTS-orf116b transgenic (H) Arabidopsis plants at seed maturity. I2-KI staining of the pollen of the wild-type (I) and CaMV::35S-orf116b-GFP transgenic (J) tobacco plants. Alexander staining of the pollen of the wild-type (K) and CaMV::35S-orf116b-GFP transgenic (L) tobacco plants. Flowers of the wild-type (M) and OE-MTS-orf116b transgenic (P) cotton plants at pollen maturity. Anthers and stigmas of the wild-type (N) and OE-MTS-orf116b transgenic (Q) cotton plants at pollen maturity. I2-KI staining of the pollen of the wild-type (O) and CaMV::35S-orf116b-GFP transgenic (R) cotton plants.