Figure 2.

Mitomycin C causes enhanced cleavage of sphingomyeline and upregulates cellular levels of the ceramide species in ASM-overexpressing cells. The ceramide levels in ASM transfected (ASM) and control transfected (pJK) Huh7.5 after treatment with 3 µM mitomycin C (MMC) for 48 h. The data are expressed in % of the untreated control and are means ± SD from a total of three independent experiments (n = 3). *** p < 0.0001, ** p < 0.001, and * p < 0.05 are considered significantly different when compared to the control group.