Figure 8.

qRT-PCR analysis of MsPUBs under abiotic stress. qRT-PCR analysis of MsPUBs under salt, drought, and cold stresses. Eleven abiotic stress-responsive genes were selected for qRT-PCR experiments. The X-axis represents control, salt treatment, drought treatment, and cold treatment. The Y-axis represents the relative expression level of MsPUB genes. The relative expression was calculated using the 2^−ΔΔCT method, with the expression level of the “control” set to 1. GAPDH was used as the internal control. The blue color represents Zhaodong and the green color represents WL525HQ. Statistical significance was determined using the t-test (** p < 0.01). Asterisks indicate significant differences.