Skip to main content
. 2024 Nov 17;25(22):12331. doi: 10.3390/ijms252212331

Figure 1.

Figure 1

Traf5−/− mice immunized with TNP-KLH/CFA exhibit attenuated antigen-specific antibody responses. (A) Experimental schema. (B) TNP-specific IgM and IgG1 antibody (Ab) responses on day 7 in sera from Traf5+/+ (WT) (n = 7) and Traf5−/− (KO) (n = 7) mice immunized with trinitrophenol-conjugated keyhole limpet hemocyanin (TNP-KLH) and complete Freund’s adjuvant (CFA) in the footpads, determined by ELISA. (C–E) Enumeration of TNP-specific IgM- and IgG1-producing cells on day 7 in popliteal draining lymph node (dLN) cells from WT (n = 11) and KO (n = 12) mice, as shown in B, determined by ELISPOT. Total numbers of dLN cells (C) and numbers of anti-TNP IgM- and IgG-producing cells in one dLN (D). Representative images of ELISPOT wells (E). The bars indicate average values for individual mice. * p < 0.05 (Student’s t-test).