Figure 3.

Traf5^−/− mice immunized with TNP-KLH/CFA exhibit normal development of Tfh and Tfr cells. The development of follicular helper T (Tfh) and follicular regulatory T (Tfr) cells in WT (n = 7) and KO (n = 7) mice, as shown in Figure 1, was evaluated by flow cytometry. (A) Gating strategy for identifying Foxp3⁻PD-1⁺CXCR5⁺CD4⁺ Tfh cells and Foxp3⁺PD-1⁺CXCR5⁺CD4⁺ Tfr cells in dLN cells from WT and KO mice. (B) Percentages and numbers of Tfh and Tfr cell populations. Bars indicate average values for individual mice. (C) Expression levels of Cd40lg in popliteal LNs from non-immunized mice and in CXCR5⁺PD-1⁺CD4⁺ T cells from popliteal LNs of mice 7 days post-immunization, as shown in Figure 1. Data are presented relative to Rn18s expression as mean ± standard deviation (n = 3), from a representative experiment of two independent experiments showing similar results. * p < 0.05 (Student’s t-test).